Evaluation of seeding techniques by monitoring cell distribution using label-free live cell imaging

Cell quality evaluationMeasure the cell density and distribution from phase contrast images and visualize cell density and its distribution with heat map style. Uniformness of cell distribution and resultant growth ability can be quantified.

Background

Proliferation activity of cells is easily affected by culture conditions and requires careful attention. Improper cell culturing skills can alter and spoil the quality and character of cells. Uniform cell distribution inside the culture vessel is  a crucial index for evaluating the cell culturing process. Although cell density in culture vessel is commonly measured by operator's visual assessment, using an objective, non-biased quantitative method to measure cell density and its distribution ensures MSC culture conditions are optimal and eliminates operator error.

Intentions

An immortalized human mesenchymal stem cells line UE7T-13 (JCRB1154) were cultured and monitored in a 6-well plate by time lapse live imaging in a culture observation system, BioStation CT. Phase contrast images were captured every 12 hours automatically.

Cell culture technician A

Cell culture technician B

Nikon provides an evaluation system for cell quality and cell processing using live cell imaging and imaging technology, contributing to stable cell manufacturing.

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