confluent
Confluence （Confluent monolayer）is when the adherent cells cover the adherent surface of the culture vessel. When culturing adherent cells, the rate of cell growth can be determined based on the ratio of the cultured cells covering the adhesion surface of the culture vessel (cell occupied area ratio or confluency).
When 70-80% of the adhesive surface of the culture vessel is occupied by cultured cells and there is still some room for cells to grow, it is called subconfluent. When the adhesive surface of the culture vessel is completely covered with cultured cells, and there is no room for cells to grow as a monolayer, it is called confluent. When confluence is reached, in normal cells, contact inhibition occurs. In other words, cell growth is inhibited by contact with cells, and the cell’s ability to grow decreases after passage. However, in some cell lines, such as immortalized cells, such contact inhibition does not occur, and an additional cell layer is formed on the cells, which may continue to proliferate. In suspension culture, as the number of cells increases, the culture medium appears turbid. When it reaches confluence, the cells aggregate and the growth rate slows down.
Typically, passage is performed at sub-confluence before confluence is reached.
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