efficiency for cell line development
Establishment refers to the creation of a cell line that allows cells to self-renew with stable characteristics. Establishment efficiency refers to the ratio of cells established to become target cells as a result of cell culture. Cells isolated from normal tissues of living organisms do not self-renew and age each time they divide, and the number of cell divisions is limited. On the other hand, cancer cells can continue to self-renew, so cell culture can be continued. ES cells (embryonic stem cells / ESCs) and iPS cells (induced pluripotent stem cells / iPSCs) can self-renew and cell culture can be continued. However, not all cancer cells can be established. Therefore, establishment efficiency is used to evaluate the operations and methods. When iPS cells are established from somatic cells, various conditions such as gene transfer efficiency have an effect, so the establishment efficiency is recognized as an important value for examining the conditions.
Share this page
