live cell imaging
Live cell imaging is a method of visualizing the movement and morphological changes of individual or collective cells, as well as the expression of genes and proteins, from the outside while the cells are alive.
Live cell imaging involves observing cells for a long time in a live state, so an incubator, a stage that can be maintained stably for a long time, and refocusing are required. Various life phenomena can be confirmed by observing with transparent images over time such as dynamic cell movement during embryonic development, cell division, cell migration of cancer cells, neurite outgrowth of nerve cells, etc. It is also used for observing the expression process of genes and proteins using fluorescent dye staining or protein staining, confirming changes over time in cells that use them as markers, and furthermore, for studying cell transport. A study of living cells by time-lapse imaging will lead to a better understanding of cell function.
When performing live cell imaging over a long period of time, care must be taken to prevent cells from getting damaged by phototoxicity.
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