Cell seeding is to spread cells to a culture vessel for cell culture activities. In the case of adherent cells, this refers to the use of a suitable material, or in some cases, the addition of a cell suspension onto a surface-treated culture vessel. When the adherent cells come into contact with the attachement factor of the culture vessel surface, they check the molecules, begin to adhere, and start to spread out on the surface.
The optimal seeding density range varies from cell to cell for both adherent and suspended cells. Therefore, when the seeding density is specified, it is often necessary to dilute cell density of cell suspension prior to seeding. If the seeding density is not specified and is unknown, it is necessary to find an appropriate seeding density range by varying the seeding density conditions and creating a growth curve.